hybridoma technique

In 1975, KO | ¨ hler and Milstein have developped hybridoma technique for producting monoclonal antibody ( McAb ).

1975年KOhler和Milstein建立了杂交瘤技术制备了单克隆抗体 ( monoclonalantibody;McAb ).

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Three hybridoma cell lines CY-4. CY-5 and CY-6 were established by hybridoma technique and the three strains of hybridoma cells could secreted monoclonal antibody against bacteroides gingivalis.

应用杂交瘤技术建立了三株分泌牙龈类杆菌单克隆抗体的细胞系CY4、CY5和CY6。

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Monoclonal antibody against delta-endotoxin protein was ra-ised by the hybridoma technique and detected by an indirect enzyme.

用纯化蛋白作为抗原免疫小鼠，通过杂交瘤技术建立了抗HD-1δ-内毒素蛋白的单克隆抗体杂交瘤细胞株。

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( Vim) isolated from pig lens. Two hybridoma cell liaes ( H1C2 and I3B2) continuously secreting anti-Vim McAb ( IgG1 subclass) were established by the hybridoma technique.

用杂交瘤技术建立了两株稳定分泌抗波形蛋白单克隆抗体（IgG1亚类）的杂交瘤细胞株：H1C2和I3B2；

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Objective: To prepare the monoclonal antibody of mouse anti-human IgG by hybridoma technique, and was applied in the preparing of the colloidal gold strip.

目的：通过杂交瘤技术，制备出鼠抗人IgG单克隆抗体，并将其运用到结核抗体IgG检测试纸条中，研制出能快速灵敏的检测出结核抗体IgG的检测试纸条。

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Two hybridoma cell lines were achieved successfully with hybridoma technique, which could stably secret specific McAb against Cys C. After one of the hybridoma cell lines was injected into mice abdominal cavity, the ascites abundant in McAb was obtained.

采用杂交瘤技术，建立了稳定分泌抗Cys C单克隆抗体的2株杂交瘤细胞株，将其中1株细胞注射于小鼠腹腔，得到了富含Cys C单克隆抗体的小鼠腹水。

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METHODS: α-ZER was conjugated to BSA as immunogen to immunize BALB/ c mice and mAb were prepared by hybridoma technique.

方法：结合O-羧甲基羟胺法和EDC法制备α-ZER-BSA偶联物，免疫BALB/c小鼠，采用杂交瘤技术制备mAb。

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Objective To prepare celluloseby hybridoma technique, and to establish I-ELISA method for clinical application.

目的利用杂交瘤技术制备抗猪囊虫单克隆抗体, 建立抑制性酶联免疫吸附法I-ELISA并应用于临床.

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Methods The Balb/ c mice were immunized with the malignant human brain glioma cell SHG-44 as antigen and the hybridoma cell line secreting anti-glioma McAb steadily was obtained by using hybridoma technique.

方法以人脑恶性胶质瘤细胞系SHG-44为抗原免疫Balb/c小鼠，通过杂交瘤技术获得稳定分泌抗胶质瘤McAb的杂交瘤细胞株。

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The principles of hybridoma technique are involved in some advantages, high specificity, sensitivity, convenience and quickness.

杂交瘤技术原理和操作过程包含了其许多优点, 特异性强 、 方便 、 敏感和快速.

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Objective To prepare monoclonal antibody ( McAb) against cysticorus cellulose antigen by hybridoma technique, and to establish I-ELISA method for clinical application.

目的利用杂交瘤技术制备抗猪囊虫单克隆抗体，建立抑制性酶联免疫吸附法I-ELISA并应用于临床。

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Methods: Monoclonal antibodies ( McAb) were prepared by hybridoma technique.

方法：采用杂交瘤技术制备单克隆抗体（McAb）。

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METHODS BALB/ c mice were immunized with human liver microsome proteins, and mAb was prepared by hybridoma technique. The crude mAb was purified by protein-G affinity chromatography.

方法：以含hCE-Ⅱ的人肝脏微粒体蛋白混合抗原免疫BALB/c小鼠，采用杂交瘤技术制备鼠抗hCE-Ⅱ的mAb，并用Protein-G亲和层析法纯化mAb。

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A hybridoma cell line ( Jc-11). which secretes a strain specific monoclonal antibody ( McAb) against Brucella Vaccine strain MS ( or M5-90), was established by lymphocyte hybridoma technique.

本实验应用淋巴细胞杂交瘤技术，建立了分泌抗布鲁氏菌弱毒疫苗M5（或M590）菌株的株系特异的单克隆抗体（McAb），杂交瘤细胞株（IC11）。

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Methods: BALB/ C mice were immunized by injecting human B lymphocytes infected with EBV cell line RPMI-8866. And preparing, purifying and evaluating the CD 23 McAb had been done with hybridoma technique, cell ELISA and Western-blotting methods.

方法：用EBV感染的人B淋巴细胞（RPMI－8866）免疫BALB／C小鼠，采用杂交瘤技术，细胞ELISA和Western－bloting等方法制备、纯化和鉴定了CD23McAb。

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[ Methods] 15 monoclonal antibodies ( McAbs) against T s muscle larva ( ML) soluble antigens were obtained by using hybridoma technique. The reactivity of monoclonal and polyclonal antibodies were tested by ELISA, Western blotting and indirect immunofluorescence assay ( IFA).

[方法]采用杂交瘤技术，获得15株特异性单克隆抗体，随后用酶联免疫吸附试验（ELISA）、免疫印迹法（WESTERN BLOTTING）和间接免疫荧光试验（IFA）对部分免疫显性抗原进行分析。

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This study aimed to establish B cell hybridoma strain secreting monoclonal antibodies ( McAb) specific to excretory-secretory ( ES) antigen of muscle larvae of Trichinella spiralis applying to hybridizable technique and the McAbs were characterized.

利用杂交瘤技术建立分泌抗旋毛虫肌幼虫排泄分泌（ES）抗原单克隆抗体（McAb）杂交瘤细胞株，并对其及分泌的McAb进行鉴定。

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